Rat HO1 (Heme Oxygenase 1) ELISA Kit

Specifications

Catalog number

E-EL-R0488-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

HO1

UniProt

P06762

Cross Reactivity

This kit recognizes Rat HO1 in samples. No significant cross-reactivity or interference between Rat HO1 and analogues was observed

Target

HO1; HMOX1; HMOX1D; HSP32; Bk286b10

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HO1. You can calculate the concentration of Rat HO1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/1673320

Citation 02

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d2fo00918h

Citation 03

https://www.hindawi.com/journals/omcl/2022/4509204/

Citation 04

https://www.sciencedirect.com/science/article/pii/S027869152200669X

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883019/

Citation 06

https://link.springer.com/article/10.1007/s10787-020-00785-5

Citation 07

https://www.sciencedirect.com/science/article/pii/S0300483X22002621

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476650/

Citation 09

https://www.sciencedirect.com/science/article/pii/S0014299921001977

Citation 10

https://www.sciencedirect.com/science/article/pii/S1319562X22002248

Citation 11

https://www.mdpi.com/1742692

Citation 12

https://www.sciencedirect.com/science/article/pii/S0024320520303295

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat HO1 concentrations in serum, plasma and other biological fluids.