Rat Hepc (Hepcidin) ELISA Kit

Specifications

Catalog number

E-EL-R0500-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Hepc

UniProt

Q99MH3

Cross Reactivity

This kit recognizes Rat Hepc in samples. No significant cross-reactivity or interference between Rat Hepc and analogues was observed

Target

Hepcidin Antimicrobial Peptide

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Hepc. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Hepc. You can calculate the concentration of Rat Hepc in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.rsc.org/en/content/articlelanding/2023/fo/d2fo02226e

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202101064

Citation 03

https://www.sciencedirect.com/science/article/pii/S0041008X19303564

Citation 04

https://www.sciencedirect.com/science/article/pii/S0001706X16305691

Citation 05

https://www.hindawi.com/journals/crp/2022/3972829/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat Hepc concentrations in serum, plasma and other biological fluids.