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Product details

Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit

Catalog number E-EL-R0520-01

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Price for E-EL-R0520-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-R0520-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
NOS2; iNOS
UniProt
Q06518
Cross Reactivity
This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed
Target
NOS2A; HEP-NOS; I-NOS
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Metabolism; Neuroscience
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS2/iNOS. You can calculate the concentration of Rat NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0753332218335200
Citation 02
https://www.mdpi.com/2076-3921/11/2/255
Citation 03
https://www.sciencedirect.com/science/article/pii/S0141813022007279
Citation 04
https://www.mdpi.com/1103930
Citation 05
https://cmjournal.biomedcentral.com/articles/10.1186/s13020-021-00562-8
Citation 06
https://www.frontiersin.org/articles/10.3389/fphar.2021.741623/full
Citation 07
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253514/
Citation 08
https://www.mdpi.com/1871692
Citation 09
https://www.sciencedirect.com/science/article/pii/S0753332220309744
Citation 10
https://journals.physiology.org/doi/abs/10.1152/ajpendo.00093.2021
Citation 11
https://www.nature.com/articles/s41598-022-09380-w
Citation 12
https://rep.bioscientifica.com/view/journals/rep/160/6/REP-20-0381.xml
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit?

The catalog number for Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is E-EL-R0520.

What sizes are available for Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit?

Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit come in?

Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is available in 3 different pack sizes.

Is Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit in stock?

Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit?

Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit?

Estimated delivery time for Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit?

The safety data sheet for Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit is available here: https://789.bio/eb/aLqn1G.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

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