Rat I-PTH (Intact Parathormone) ELISA Kit

Specifications

Catalog number

E-EL-R0535-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

I-PTH

UniProt

P04089

Cross Reactivity

This kit recognizes Rat I-PTH in samples. No significant cross-reactivity or interference between Rat I-PTH and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat I-PTH. You can calculate the concentration of Rat I-PTH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01733-4

Citation 02

https://www.sciencedirect.com/science/article/pii/S0024320522006282

Citation 03

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03867b

Citation 04

https://www.frontiersin.org/articles/10.3389/fphys.2023.1083725/full?utm_source=dlvr.it&utm_medium=twitter

Citation 05

https://www.hindawi.com/journals/omcl/2019/2370862/abs/

Citation 06

https://www.frontiersin.org/articles/10.3389/fphar.2020.00392/full?report=reader

Citation 07

https://www.sciencedirect.com/science/article/pii/S8756328220303422

Citation 08

https://link.springer.com/article/10.1007/s10534-022-00449-7

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039685/

Citation 10

https://link.springer.com/article/10.1007/s12020-022-03199-1

Citation 11

https://www.spandidos-publications.com/10.3892/etm.2018.6528

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat I-PTH concentrations in serum, plasma and other biological fluids.