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Product details

Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit

Catalog number E-EL-R0540-01

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Price for E-EL-R0540-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-R0540-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
TIMP-1
UniProt
P30120
Cross Reactivity
This kit recognizes Rat TIMP-1 in samples. No significant cross-reactivity or interference between Rat TIMP-1 and analogues was observed
Target
TIMP1; CLGI; EPA; EPO; HCI
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cell Biology; Cardiovascular; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TIMP-1. You can calculate the concentration of Rat TIMP-1 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.1 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0753332222003377
Citation 02
https://www.sciencedirect.com/science/article/pii/S0378874122000563
Citation 03
https://www.hindawi.com/journals/mi/2020/4260204/
Citation 04
https://www.sciencedirect.com/science/article/pii/S0946672X2100136X
Citation 05
https://onlinelibrary.wiley.com/doi/abs/10.1111/aji.13096
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat TIMP-1 concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit?

The catalog number for Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is E-EL-R0540.

What sizes are available for Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit?

Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit come in?

Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is available in 3 different pack sizes.

Is Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit in stock?

Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit?

Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit?

Estimated delivery time for Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit?

The safety data sheet for Rat TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit is available here: https://789.bio/eb/D8yDy9.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

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