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Product details

Rat FN (Fibronectin) ELISA Kit

Catalog number E-EL-R0578-01

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Price for E-EL-R0578-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-R0578-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
FN
UniProt
P04937
Cross Reactivity
This kit recognizes Rat FN in samples. No significant cross-reactivity or interference between Rat FN and analogues was observed
Target
FN1; CIG; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cardiovascular; Developmental biology; Signal transduction; Stem cells
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FN. You can calculate the concentration of Rat FN in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.25-80 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.75 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.sciencedirect.com/science/article/pii/S0024320518304351
Citation 02
https://link.springer.com/article/10.1007/s12012-021-09643-4
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat FN concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Rat FN (Fibronectin) ELISA Kit?

The catalog number for Rat FN (Fibronectin) ELISA Kit is E-EL-R0578.

What sizes are available for Rat FN (Fibronectin) ELISA Kit?

Rat FN (Fibronectin) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Rat FN (Fibronectin) ELISA Kit come in?

Rat FN (Fibronectin) ELISA Kit is available in 3 different pack sizes.

Is Rat FN (Fibronectin) ELISA Kit in stock?

Rat FN (Fibronectin) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Rat FN (Fibronectin) ELISA Kit?

Rat FN (Fibronectin) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Rat FN (Fibronectin) ELISA Kit?

Estimated delivery time for Rat FN (Fibronectin) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Rat FN (Fibronectin) ELISA Kit?

The safety data sheet for Rat FN (Fibronectin) ELISA Kit is available here: https://789.bio/eb/unfbbL.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

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