Rat NFKB-p65 (Nuclear Factor Kappa B p65) ELISA Kit

Specifications

Catalog number

E-EL-R0674-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

NFKB-p65

UniProt

O88619

Cross Reactivity

This kit recognizes Rat NFKB-p65 in samples. No significant cross-reactivity or interference between Rat NFKB-p65 and analogues was observed

Target

NFKB3; p65; RelA

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Microbiology; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFKB-p65. You can calculate the concentration of Rat NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332222004450

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332221009859

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332221001967

Citation 04

https://cancerci.biomedcentral.com/articles/10.1186/s12935-022-02759-9

Citation 05

https://www.sciencedirect.com/science/article/pii/S0024320522006397

Citation 06

https://www.sciencedirect.com/science/article/pii/S002432052200577X

Citation 07

https://www.mdpi.com/1871798

Citation 08

https://www.sciencedirect.com/science/article/pii/S002432052200981X

Citation 09

https://www.sciencedirect.com/science/article/pii/S0889159120303524

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429807/

Citation 11

https://europepmc.org/articles/pmc9424734/bin/table5.docx?javascript_support=no

Citation 12

https://www.mdpi.com/2218-1989/12/7/648

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p65 concentrations in serum, plasma and other biological fluids.