Rat PTX-3/TSG-14 (Pentraxin 3) ELISA Kit

Specifications

Catalog number

E-EL-R1002-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

PTX-3; TSG-14

UniProt

P81062

Cross Reactivity

This kit recognizes Rat PTX-3/TSG-14 in samples. No significant cross-reactivity or interference between Rat PTX-3/TSG-14 and analogues was observed

Target

PTX3; TNFAIP5; TSG-14; Pentraxin 3; long; TNF-inducible gene 14 protein

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PTX-3/TSG-14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTX-3/TSG-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTX-3/TSG-14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PTX-3/TSG-14. You can calculate the concentration of Rat PTX-3/TSG-14 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S075333222100473X

Citation 02

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03403k

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat PTX-3/TSG-14 concentrations in serum, plasma and other biological fluids.