Rat VCAM-1/CD106 (Vascular Cell Adhesion Molecule 1) ELISA Kit

Specifications

Catalog number

E-EL-R1061-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

VCAM-1; CD106

UniProt

P29534

Cross Reactivity

This kit recognizes Rat VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Rat VCAM-1/CD106 and analogues was observed

Target

CD106; INCAM-100

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VCAM-1/CD106. You can calculate the concentration of Rat VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

12.5-800 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

7.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://ard.bmj.com/content/81/Suppl_1/496.2.abstract

Citation 02

https://www.sciencedirect.com/science/article/pii/S0007091221000908

Citation 03

https://www.mdpi.com/1422-0067/23/16/8883

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/13510002.2022.2129192

Citation 05

https://www.nature.com/articles/srep33390

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332220312555

Citation 07

https://www.sciencedirect.com/science/article/pii/S001429992100707X

Citation 08

https://www.frontiersin.org/articles/10.3389/fphys.2017.00949/full

Citation 09

https://www.sciencedirect.com/science/article/pii/S0014299919307381

Citation 10

https://journals.sagepub.com/doi/abs/10.1177/1535370220961038

Citation 11

https://www.frontiersin.org/articles/10.3389/fphys.2018.00020/full

Citation 12

https://link.springer.com/article/10.1007/s00210-019-01630-5

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.