Rat SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit

Specifications

Catalog number

E-EL-R1424-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SOD1

UniProt

P07632

Cross Reactivity

This kit recognizes Rat SOD1 in samples.No significant cross-reactivity or interference between Rat SOD1 and analogues was observed

Target

ALS; ALS1; IPOA; Superoxide dismutase [Cu-Zn]

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat SOD1. During the reaction, Rat SOD1 in the sample or standard competes with a fixed amount of Rat SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/1278460

Citation 02

https://www.sciencedirect.com/science/article/pii/S075333222200614X

Citation 03

https://www.tandfonline.com/doi/abs/10.1080/15476286.2019.1657352

Citation 04

https://pubmed.ncbi.nlm.nih.gov/34776947/

Citation 05

https://academic.oup.com/ijnp/article-abstract/22/1/57/5164721

Citation 06

https://www.mdpi.com/2072-6643/12/11/3511

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.15351

Citation 08

https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/biof.1692

Citation 09

https://link.springer.com/article/10.1007/s11356-022-18626-z

Citation 10

https://www.sciencedirect.com/science/article/pii/S0304394021007643

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8542477/

Citation 12

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257177

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat SOD1 concentrations in serum, plasma and other biological fluids.