Rat ET-1 (Endothelin 1) ELISA Kit

Specifications

Catalog number

E-EL-R1458-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ET-1

UniProt

P22388

Cross Reactivity

This kit recognizes Rat ET-1 in samples.No significant cross-reactivity or interference between Rat ET-1 and analogues was observed

Target

EDN1; ET1; HDLCQ7; PPET1; Preproendothelin-1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ET-1. You can calculate the concentration of Rat ET-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S075333222300118X

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7901921/

Citation 03

https://www.nature.com/articles/srep33390

Citation 04

https://journals.lww.com/jhypertension/Fulltext/2022/04000/Rho_kinase_inhibition_ameliorates_vascular.7.aspx

Citation 05

https://www.sciencedirect.com/science/article/pii/S075333222030593X

Citation 06

https://www.sciencedirect.com/science/article/pii/S0939475322004227

Citation 07

https://www.spandidos-publications.com/10.3892/ijmm.2021.5008

Citation 08

https://www.sciencedirect.com/science/article/pii/S0378874120332967

Citation 09

https://www.spandidos-publications.com/10.3892/ijmm.2020.4567

Citation 10

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151005

Citation 11

https://content.iospress.com/articles/journal-of-alzheimers-disease/jad190630

Citation 12

https://www.hindawi.com/journals/mi/2020/8387654/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat ET-1 concentrations in serum, plasma and other biological fluids.