Rat GPX1 (Glutathione Peroxidase 1) ELISA Kit

Specifications

Catalog number

E-EL-R2491-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

GPX1

UniProt

P04041

Cross Reactivity

This kit recognizes Rat GPX1 in samples. No significant cross-reactivity or interference between Rat GPX1 and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GPX1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GPX1. You can calculate the concentration of Rat GPX1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubmed.ncbi.nlm.nih.gov/36330218/

Citation 02

https://www.mdpi.com/article/10.3390/ijms232315400

Citation 03

https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-021-00573-0

Citation 04

https://link.springer.com/article/10.1007/s11356-022-20593-4

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/jdi.13690

Citation 06

https://www.nature.com/articles/s41598-020-72098-0

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8542477/

Citation 08

https://www.hindawi.com/journals/jdr/2019/6753541/abs/

Citation 09

https://www.sciencedirect.com/science/article/pii/S1382668920300636

Citation 10

https://www.sciencedirect.com/science/article/pii/S0300483X18300994

Citation 11

https://www.sciencedirect.com/science/article/pii/S0753332219307577

Citation 12

https://www.sciencedirect.com/science/article/pii/S1319016419301628

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat GPX1 concentrations in serum, plasma and other biological fluids.