Rat KISS1 (Kisspeptin 1) ELISA Kit

Specifications

Catalog number

E-EL-R2530-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

KISS1

UniProt

Q7TSB7

Cross Reactivity

This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat KISS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KISS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KISS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat KISS1. You can calculate the concentration of Rat KISS1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.frontiersin.org/articles/10.3389/fphys.2022.998446/full?utm_source=dlvr.it&utm_medium=twitter

Citation 02

https://www.sciencedirect.com/science/article/pii/S0378427421002058

Citation 03

https://link.springer.com/article/10.1007/s12020-022-03191-9

Citation 04

https://link.springer.com/article/10.1007/s12020-022-03010-1

Citation 05

https://www.sciencedirect.com/science/article/pii/S0378427419303480

Citation 06

https://www.sciencedirect.com/science/article/pii/S0031938421000330

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat KISS1 concentrations in serum, plasma and other biological fluids.