Rat LDHA (Lactate Dehydrogenase A) ELISA Kit

Specifications

Catalog number

E-EL-R2547-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

LDHA

UniProt

P04642

Cross Reactivity

This kit recognizes Rat LDHA in samples. No significant cross-reactivity or interference between Rat LDHA and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LDHA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LDHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LDHA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LDHA. You can calculate the concentration of Rat LDHA in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41598-021-93234-4

Citation 02

https://www.mdpi.com/1490504

Citation 03

https://www.sciencedirect.com/science/article/pii/S0024320520316295

Citation 04

https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/evidence-based-mechanistic-role-of-chrysin-towards-protection-of-cardiac-hypertrophy-fibrosis-in-rats/F981072347E66679932F162E17722989

Citation 05

https://link.springer.com/article/10.1007/s43450-021-00216-8

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat LDHA concentrations in serum, plasma and other biological fluids.