Rat CTXII (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit

Specifications

Catalog number

E-EL-R2554-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

CTXII

UniProt

P05539 (COl2A1)

Cross Reactivity

This kit recognizes Rat CTXII in samples. No significant cross-reactivity or interference between Rat CTXII and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXII. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXII and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXII, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTXII. You can calculate the concentration of Rat CTXII in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066007/

Citation 02

https://www.mdpi.com/2072-6643/12/4/956

Citation 03

https://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01125h

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.6366

Citation 05

https://link.springer.com/article/10.1007/s10787-017-0432-2

Citation 06

https://link.springer.com/article/10.1007/s10787-018-0452-6

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.5949

Citation 08

https://www.ingentaconnect.com/content/wk/gme/2017/00000024/00000009/art00014

Citation 09

https://www.sciencedirect.com/science/article/pii/S0165247818301469

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.12755

Citation 11

https://www.spandidos-publications.com/10.3892/etm.2019.8189

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/bem.22028

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CTXII concentrations in serum, plasma and other biological fluids.