Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Specifications

Catalog number

E-EL-R2856-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TNF-α

UniProt

P16599

Cross Reactivity

This kit recognizes Rat TNF-α in samples.No significant cross-reactivity or interference between Rat TNF-α and analogues was observed

Target

DIF; TNF-alpha; TNFA; TNFSF2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Signal transduction; Metabolism; Cancer; Immunology; Microbiology; Cardiovascular

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNF-α. You can calculate the concentration of Rat TNF-α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://ccforum.biomedcentral.com/articles/10.1186/s13054-022-04184-w?fbclid=IwAR2aDS2jcwQZ5oCYKCG_kSexf9HkjoZ-XqOnLhIKn6AD-hyTWD3-Bms2vuw

Citation 02

https://www.sciencedirect.com/science/article/pii/S0142961221003082

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201661

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201680

Citation 05

https://pubs.acs.org/doi/abs/10.1021/acs.chemmater.2c01300

Citation 06

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01277-0

Citation 07

https://www.sciencedirect.com/science/article/pii/S2352940722001962

Citation 08

https://link.springer.com/content/pdf/10.1007/s00018-016-2426-4.pdf

Citation 09

https://pubs.acs.org/doi/abs/10.1021/acsami.1c03589

Citation 10

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-023-02715-y

Citation 11

https://bpspubs.onlinelibrary.wiley.com/doi/abs/10.1111/bph.15895

Citation 12

https://link.springer.com/article/10.1186/s12974-021-02303-y

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat TNF-α concentrations in serum, plasma and other biological fluids.