Rat OPG (Osteoprotegerin) ELISA Kit

Specifications

Catalog number

E-EL-R3005-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

OPG

UniProt

O08727

Cross Reactivity

This kit recognizes Rat OPG in samples.No significant cross-reactivity or interference between Rat OPG and analogues was observed

Target

TNFRSF11B; OCIF; TR1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OPG. You can calculate the concentration of Rat OPG in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450458/

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579055/

Citation 03

https://www.mdpi.com/2072-6643/10/7/799

Citation 04

https://www.liebertpub.com/doi/abs/10.1089/scd.2020.0163

Citation 05

https://link.springer.com/article/10.1007/s10787-017-0432-2

Citation 06

https://www.tandfonline.com/doi/abs/10.1080/13880209.2021.1876100

Citation 07

https://peerj.com/articles/11547/

Citation 08

https://www.ingentaconnect.com/content/wk/gme/2018/00000025/00000002/art00014

Citation 09

https://www.mdpi.com/1937306

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat OPG concentrations in serum, plasma and other biological fluids.