Rat ADP/Acrp30 (Adiponectin) ELISA Kit

Specifications

Catalog number

E-EL-R3012-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ADP; Acrp30

UniProt

Q8K3R4

Cross Reactivity

This kit recognizes Rat ADP/Acrp30 in samples.No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed

Target

Acrp30; GBP28; ACDC; APM1; ADPN; AdipoQ; ADIPQTL1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. You can calculate the concentration of Rat ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/1999-4923/14/7/1368

Citation 02

https://www.mdpi.com/1871912

Citation 03

https://www.mdpi.com/1414304

Citation 04

https://www.mdpi.com/989394

Citation 05

https://www.mdpi.com/2076-3921/9/11/1073

Citation 06

https://www.mdpi.com/1422-0067/22/10/5400

Citation 07

https://pubmed.ncbi.nlm.nih.gov/34305591/

Citation 08

https://www.frontiersin.org/articles/10.3389/fcimb.2021.740236/full

Citation 09

https://www.sciencedirect.com/science/article/pii/S0960076022001303

Citation 10

https://www.sciencedirect.com/science/article/pii/S0899900722003264

Citation 11

https://www.mdpi.com/2131052

Citation 12

https://www.sciencedirect.com/science/article/pii/S0955286320304903

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in serum, plasma and other biological fluids.