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Product details

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit

Catalog number E-EL-R3017-01

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Price for E-EL-R3017-01

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Size / count 24 Tests

Specifications

Catalog number
E-EL-R3017-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
IGFBP-3
UniProt
P15473
Cross Reactivity
This kit recognizes Rat IGFBP-3 in samples.No significant cross-reactivity or interference between Rat IGFBP-3 and analogues was observed
Target
IGFBP3; BP-53; IBP3
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cell Biology; Cardiovascular; Metabolism; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IGFBP-3. You can calculate the concentration of Rat IGFBP-3 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.14397
Citation 02
https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8167496/
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat IGFBP-3 concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit?

The catalog number for Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit is E-EL-R3017.

What sizes are available for Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit?

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit come in?

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit is available in 3 different pack sizes.

Is Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit in stock?

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit?

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit?

Estimated delivery time for Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit?

The safety data sheet for Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit is available here: https://789.bio/eb/vjD0KG.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

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