Rat AMH (Anti-Mullerian Hormone) ELISA Kit

Specifications

Catalog number

E-EL-R3022-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

AMH

UniProt

P49000

Cross Reactivity

This kit recognizes Rat AMH in samples.No significant cross-reactivity or interference between Rat AMH and analogues was observed

Target

MIF; MIH; MIS

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AMH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AMH. You can calculate the concentration of Rat AMH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0144861722002089

Citation 02

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-03056-y

Citation 03

https://www.sciencedirect.com/science/article/pii/S014765132200656X

Citation 04

https://www.sciencedirect.com/science/article/pii/S0024320522009833

Citation 05

https://link.springer.com/article/10.1186/s13287-022-02772-9

Citation 06

https://www.mdpi.com/article/10.3390/ijms232416009

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7993663/

Citation 08

https://link.springer.com/article/10.1186/s13287-020-02002-0

Citation 09

https://www.mdpi.com/2076-3921/9/7/591

Citation 10

https://www.sciencedirect.com/science/article/pii/S0753332220307046

Citation 11

https://www.liebertpub.com/doi/abs/10.1089/can.2022.0242

Citation 12

https://link.springer.com/article/10.1186/s13048-020-00763-z

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat AMH concentrations in serum, plasma and other biological fluids.