Rat SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit

Specifications

Catalog number

E-EL-R3027-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SDF-1; CXCL12

UniProt

Q9QZD1

Cross Reactivity

This kit recognizes Rat SDF-1/CXCL12 in samples.No significant cross-reactivity or interference between Rat SDF-1/CXCL12 and analogues was observed

Target

SDF1; CXCL12; IRH; PBSF; SCYB12; TLSF; TPAR1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Microbiology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDF-1/CXCL12. You can calculate the concentration of Rat SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-02736-z

Citation 02

https://stemcellsjournals.onlinelibrary.wiley.com/doi/abs/10.1002/sctm.19-0013

Citation 03

https://www.mdpi.com/1424-8247/15/7/852

Citation 04

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1353-3

Citation 05

https://www.sciencedirect.com/science/article/pii/S0014488618304461

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.15678

Citation 07

https://jcs.biologists.org/content/131/13/jcs216135.short

Citation 08

https://www.spandidos-publications.com/ijmm/36/5/1205?text=fulltext

Citation 09

https://www.sciencedirect.com/science/article/pii/S0003986119303157

Citation 10

https://rep.bioscientifica.com/view/journals/rep/161/2/REP-20-0451.xml

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.