Rat FGF23 (Fibroblast Growth Factor 23) ELISA Kit

Specifications

Catalog number

E-EL-R3031-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

FGF23

UniProt

Q8VI82

Cross Reactivity

This kit recognizes Rat FGF23 in samples.No significant cross-reactivity or interference between Rat FGF23 and analogues was observed

Target

FGF-23; ADHR; FGFN; HYPF; HPDR2; PHPTC

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FGF23. You can calculate the concentration of Rat FGF23 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03867b

Citation 02

https://www.frontiersin.org/articles/10.3389/fphys.2023.1083725/full?utm_source=dlvr.it&utm_medium=twitter

Citation 03

https://www.sciencedirect.com/science/article/pii/S2225411023000214

Citation 04

https://link.springer.com/article/10.1007/s12020-022-03199-1

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat FGF23 concentrations in serum, plasma and other biological fluids.