Rat SELP (P-Selectin) ELISA Kit

Specifications

Catalog number

E-EL-R3045-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SELP

UniProt

P98106

Cross Reactivity

This kit recognizes Rat SELP in samples.No significant cross-reactivity or interference between Rat SELP and analogues was observed

Target

IL1R2; CD121b; CDw121b; IL-1R-2; IL-1RT-2; IL-1RT2; IL1R2c; IL1RB

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SELP. You can calculate the concentration of Rat SELP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S001429992100707X

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332218344548

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acsomega.0c05227

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/08916934.2019.1575820

Citation 05

https://link.springer.com/article/10.1007/s11033-020-05746-3

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat SELP concentrations in serum, plasma and other biological fluids.