Rabbit TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Specifications

Catalog number

E-EL-RB0011-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

TNF-α

UniProt

P04924

Cross Reactivity

This kit recognizes Rabbit TNF-α in samples. No significant cross-reactivity or interference between Rabbit TNF-α and analogues was observed

Target

DIF; TNF-alpha; TNFA; TNFSF2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit TNF-α. You can calculate the concentration of Rabbit TNF-α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2076-393X/8/1/129

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201191/

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904861/

Citation 04

https://www.mdpi.com/2072-6651/10/7/289

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/aor.12658

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332217342154

Citation 07

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224052

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407652/

Citation 09

https://www.mdpi.com/2076-2615/10/9/1540

Citation 10

https://www.spandidos-publications.com/10.3892/ijmm.2016.2586?text=fulltext

Citation 11

https://www.sciencedirect.com/science/article/pii/S1878875018328924

Citation 12

https://www.sciencedirect.com/science/article/pii/S0093691X17305320

Species

Rabbit

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rabbit TNF-α concentrations in serum, plasma and other biological fluids.