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Product details

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Catalog number E-HSEL-R0001-01

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Price for E-HSEL-R0001-01

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Size / count 24 Tests

Specifications

Catalog number
E-HSEL-R0001-01
Size / count
24 Tests
Dry ice
No
Hazardous
No
Discontinued
No
Abbreviation
TNF-α
UniProt
P16599
Cross Reactivity
This kit recognizes Rat TNF-α in samples. No significant cross-reactivity or interference between Rat TNF-α and analogues was observed.
Target
DIF; TNF-alpha; TNFA; TNFSF2
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology; Microbiology; Cancer; Cardiovascular; Metabolism; Neuroscience
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TNF-α. You can calculate the concentration of Rat TNF-α in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.86 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Species
Rat
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Rat TNF-α concentrations in serum, plasma and other biological fluids.

Frequently Asked Questions

What is the catalog number for High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

The catalog number for High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is E-HSEL-R0001.

What sizes are available for High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available in the following size(s): 24 Tests, 48 Tests, 96 Tests.

How many pack sizes does High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit come in?

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available in 3 different pack sizes.

Is High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit in stock?

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is manufactured by Elabscience.

What is the estimated delivery time for High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

Estimated delivery time for High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit?

The safety data sheet for High Sensitivity Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is available here: https://789.bio/eb/Oi5SuP.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

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