Mini Sample Mouse IGF-1 (Insulin-like Growth Factor 1) ELISA Kit

Specifications

Catalog number

E-MSEL-M0013-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IGF-1

UniProt

P05017

Cross Reactivity

This kit recognizes Mouse IGF-1 in samples.No significant cross-reactivity or interference between Mouse IGF-1 and analogues was observed

Target

IGF-1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGF-1. You can calculate the concentration of Mouse IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.25-80 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.75 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0955286322002868

Citation 02

https://www.mdpi.com/2072-6694/12/3/623

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675689/

Citation 04

https://link.springer.com/article/10.1186/s12885-018-4271-3

Citation 05

https://journals.sagepub.com/doi/abs/10.1177/1559325820914172

Citation 06

https://www.sciencedirect.com/science/article/pii/S0143400415300345

Citation 07

https://www.hindawi.com/journals/aps/2020/8886894/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IGF-1 concentrations in serum, plasma and other biological fluids.