Mini Sample Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit

Specifications

Catalog number

E-MSEL-M0022-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

GM-CSF

UniProt

P01587

Cross Reactivity

This kit recognizes Mouse GM-CSF in samples.No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed

Target

GM-CSF

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GM-CSF. You can calculate the concentration of Mouse GM-CSF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.67 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2452199X21003510

Citation 02

https://pubmed.ncbi.nlm.nih.gov/35860242/

Citation 03

https://www.sciencedirect.com/science/article/pii/S1226845320301676

Citation 04

https://pubmed.ncbi.nlm.nih.gov/35153740/

Citation 05

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.201902910RRR

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874121001926

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1002/cbdv.202100139

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse GM-CSF concentrations in serum, plasma and other biological fluids.