Mini Sample Mouse SELP (P-Selectin) ELISA Kit

Specifications

Catalog number

E-MSEL-M0029-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

SELP

UniProt

Q01102

Cross Reactivity

This kit recognizes Mouse SELP in samples.No significant cross-reactivity or interference between Mouse SELP and analogues was observed

Target

SELP

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELP. You can calculate the concentration of Mouse SELP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.09 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1742706122004986

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 03

https://www.mdpi.com/2072-6694/12/3/623

Citation 04

https://www.sciencedirect.com/science/article/pii/S1043661819301124

Citation 05

https://www.hindawi.com/journals/omcl/2020/8430465/

Citation 06

https://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.8b00781

Citation 07

https://www.physiology.org/doi/abs/10.1152/ajprenal.00405.2018

Citation 08

https://journals.lww.com/cardiovascularpharm/Fulltext/2018/07000/NaoXinTong_Capsule_Inhibits_Carrageenan_Induced.9.aspx

Citation 09

https://journals.sagepub.com/doi/abs/10.1177/15593258211016257

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse SELP concentrations in serum, plasma and other biological fluids.