QuicKey Pro Bovine E2 (Estradiol) ELISA Kit

Specifications

Catalog number

E-OSEL-B0001-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

E2

Cross Reactivity

This kit recognizes Bovine E2 in samples.No significant cross-reactivity or interference between Bovine E2 and analogues was observed

Target

Oestradiol; E2;17β-Estradiol; Estra-1;3;5 (10) -triene-3;17β-diol

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell biology; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine E2 are added to the micro ELISA plate wells. Bovine E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Bovine E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

5.87 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Species

Bovine

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Bovine E2 concentrations in serum, plasma.