QuicKey Pro Human IL-6 (Interleukin 6) ELISA Kit

Specifications

Catalog number

E-OSEL-H0001-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IL-6

UniProt

P05231

Cross Reactivity

This kit recognizes Human IL-6 in samples.No significant cross-reactivity or interference between Human IL-6 and analogues was observed

Target

Interleukin 6; IL6; BSF2; HGF; HSF; IFNB2; CDF

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Immunology; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-6.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IL-6. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

3.13-200 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.02 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-021-01369-9

Citation 02

https://aasldpubs.onlinelibrary.wiley.com/doi/abs/10.1002/hep.31056

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acsnano.6b06658

Citation 04

https://link.springer.com/article/10.1007/s42765-022-00252-6

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0048969722056844

Citation 07

https://www.sciencedirect.com/science/article/pii/S0049384822004807

Citation 08

https://link.springer.com/article/10.1186/s12951-022-01288-x

Citation 09

https://pubs.acs.org/doi/abs/10.1021/acsami.2c16418

Citation 10

https://www.sciencedirect.com/science/article/pii/S0269749122014312

Citation 11

https://link.springer.com/article/10.1007/s00018-021-03902-x

Citation 12

https://pubs.acs.org/doi/abs/10.1021/acsami.9b04827

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma.