QuicKey Pro Human FE (Ferritin) ELISA Kit

Specifications

Catalog number

E-OSEL-H0003-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

FE

UniProt

P02792, P02794

Cross Reactivity

This kit recognizes Human FE in samples.No significant cross-reactivity or interference between Human FE and analogues was observed

Target

Ferritin; FE

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human FE are added to the micro ELISA plate wells. Human FE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human FE.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human FE. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

24.31 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://academic.oup.com/jcem/article-abstract/105/4/e1056/5733667

Citation 02

https://www.tandfonline.com/doi/abs/10.1080/07853890.2022.2157475

Citation 03

https://www.mdpi.com/1458308

Citation 04

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265772

Citation 05

https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/vitamin-d3-supplementation-for-8-weeks-leads-to-improved-haematological-status-following-the-consumption-of-an-ironfortified-breakfast-cereal-a-doubleblind-randomised-controlled-trial-in-irondeficient-women/BF1FD0291A110FF2D488064EF4A2BF40

Citation 06

https://www.sciencedirect.com/science/article/pii/S0946672X21000031

Citation 07

https://link.springer.com/article/10.1186/s12902-017-0204-4

Citation 08

https://www.sciencedirect.com/science/article/pii/S1658361222000919

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human FE concentrations in serum, plasma.