QuicKey Pro Human AMH (Anti-Mullerian Hormone) ELISA Kit

Specifications

Catalog number

E-OSEL-H0004-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

7732-18-5

Abbreviation

AMH

Synonyms

AMH; Anti-Mullerian Hormone;

UniProt

P03971

Species Reactivity

Human

Cross Reactivity

This kit recognizes Human AMH in samples.No significant cross-reactivity or interference between Human AMH and analogues was observed

Target

Human

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction; Stem cells

Detection Method

Colormetric

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AMH. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human AMH are added to the micro ELISA plate wells. Human AMH in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human AMH.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human AMH. The concentration of Human AMH in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%

Recovery

80%-120%

Sensitivity

46.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C, 6 months

Applications Notes

This ELISA kit applies to the in vitro quantitative determination of Human AMH concentrations in serum, plasma.

Citation 01

https://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqac042/6580263