QuicKey Pro Human E2 (Estradiol) ELISA Kit

Specifications

Catalog number

E-OSEL-H0005-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

E2

Cross Reactivity

This kit recognizes Human E2 in samples.No significant cross-reactivity or interference between Human E2 and analogues was observed

Target

Oestradiol; E2;17β-Estradiol; Estra-1;3;5 (10) -triene-3;17β-diol

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell biology; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E2 are added to the micro ELISA plate wells. Human E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

13.22 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S030438942102447X

Citation 02

https://www.sciencedirect.com/science/article/pii/S0304389420306002

Citation 03

https://www.nature.com/articles/s12276-020-00491-w

Citation 04

https://www.nature.com/articles/s41598-021-87583-3

Citation 05

https://www.nature.com/articles/s41598-021-87583-3

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378427421009024

Citation 07

https://www.sciencedirect.com/science/article/pii/S0041008X1930016X

Citation 08

https://rep.bioscientifica.com/view/journals/rep/162/1/REP-20-0643.xml

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1002/jat.3783

Citation 10

https://www.ingentaconnect.com/content/asp/asl/2018/00000024/00000008/art00150

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human E2 concentrations in serum, plasma, saliva, urine.