QuicKey Pro Human IgA (Immunoglobulin A) ELISA Kit

Specifications

Catalog number

E-OSEL-H0009-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IgA

Cross Reactivity

This kit recognizes Human IgA in samples.No significant cross-reactivity or interference between Human IgA and analogues was observed

Target

Immunoglobulin A; IgA

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IgA are added to the micro ELISA plate wells. Human IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IgA.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IgA. The concentration of Human IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, Plasma, Urine, Saliva

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.14 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.mdpi.com/1457378

Citation 02

https://www.mdpi.com/2304-8158/11/5/759

Citation 03

https://www.mdpi.com/2304-8158/10/7/1580

Citation 04

https://www.frontiersin.org/articles/10.3389/fphar.2020.588560/full?utm_source=researcher_app&utm_medium=referral&utm_campaign=RESR_MRKT_Researcher_inbound

Citation 05

https://www.frontiersin.org/articles/10.3389/fmicb.2018.01403/full

Citation 06

https://www.mdpi.com/article/10.3390/microorganisms10061256

Citation 07

https://www.frontiersin.org/articles/10.3389/fphys.2021.735986/full

Citation 08

https://www.mdpi.com/2076-2607/10/7/1453

Citation 09

https://link.springer.com/article/10.1007/s11010-021-04070-z

Citation 10

https://www.sciencedirect.com/science/article/pii/S1876034121003993

Citation 11

https://www.sciencedirect.com/science/article/pii/S0032579119440923

Citation 12

https://www.tandfonline.com/doi/abs/10.1080/14767058.2018.1455089

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IgA concentrations in serum, plasma, saliva, urine.