QuicKey Pro Human D2D (D-Dimer) ELISA Kit

Specifications

Catalog number

E-OSEL-H0010-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

D2D

Cross Reactivity

This kit recognizes Human D2D in samples.No significant cross-reactivity or interference between Human D2D and analogues was observed

Target

D-Dimer; D dimer; D2D

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cardiovascular

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human D2D are added to the micro ELISA plate wells. Human D2D in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human D2D.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human D2D. The concentration of Human D2D in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Plasma

Detection Range

125-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

53.1 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1931524417301226

Citation 02

https://www.sciencedirect.com/science/article/pii/S1567576920314946

Citation 03

https://pubmed.ncbi.nlm.nih.gov/33577011/

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human D2D concentrations in plasma.