QuicKey Pro Human Pg (Progesterone) ELISA Kit

Specifications

Catalog number

E-OSEL-H0011-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Pg

Cross Reactivity

This kit recognizes Human Pg in samples.No significant cross-reactivity or interference between Human Pg and analogues was observed

Target

Progesterone; Pg

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Pg are added to the micro ELISA plate wells. Human Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

36.24 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.tandfonline.com/doi/abs/10.1080/20013078.2020.1795364

Citation 02

https://www.sciencedirect.com/science/article/pii/S016503782200167X

Citation 03

https://www.jbc.org/content/291/29/15108.short

Citation 04

https://www.sciencedirect.com/science/article/pii/S0378874121000969

Citation 05

https://www.sciencedirect.com/science/article/pii/S0378427421009024

Citation 06

https://www.sciencedirect.com/science/article/pii/S0887233321002277

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human Pg concentrations in serum, plasma.