QuicKey Pro Human POSTN/OSF-2 (Periostin) ELISA Kit

Specifications

Catalog number

E-OSEL-H0013-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

POSTN; OSF-2

UniProt

Q15063

Cross Reactivity

This kit recognizes Human POSTN/OSF-2 in samples.No significant cross-reactivity or interference between Human POSTN/OSF-2 and analogues was observed

Target

POSTN/OSF-2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Signal transduction; Tags and Cell Markers

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human POSTN/OSF-2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human POSTN/OSF-2. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

3.12-200 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.52 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://link.springer.com/article/10.1007/s12020-022-03027-6

Citation 02

https://link.springer.com/article/10.1007/s00784-022-04681-w

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/ijcp.14728

Citation 04

https://link.springer.com/article/10.1007/s10157-019-01744-3

Citation 05

https://www.tandfonline.com/doi/abs/10.1080/20469047.2019.1682330

Citation 06

https://www.degruyter.com/document/doi/10.1515/tjb-2022-0081/html

Citation 07

https://www.sciencedirect.com/science/article/pii/S2213398421001780

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human POSTN/OSF-2 concentrations in serum, plasma.