QuicKey Pro Human IL-8 (Interleukin 8) ELISA Kit

Specifications

Catalog number

E-OSEL-H0014-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IL-8

UniProt

P10145

Cross Reactivity

This kit recognizes Human IL-8 in samples.No significant cross-reactivity or interference between Human IL-8 and analogues was observed

Target

IL8/CXCL8

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-8 are added to the micro ELISA plate wells. Human IL-8 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-8.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IL-8. The concentration of Human IL-8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S009286742031000X

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120224/

Citation 03

https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-022-02535-6

Citation 04

https://www.sciencedirect.com/science/article/pii/S0304383522000040

Citation 05

https://www.nature.com/articles/s41419-022-04734-8

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351451/

Citation 07

https://www.nature.com/articles/s41416-022-01744-3

Citation 08

https://www.mdpi.com/2072-6694/15/5/1510

Citation 09

https://www.frontiersin.org/articles/10.3389/fcell.2020.574940/full

Citation 10

https://www.sciencedirect.com/science/article/pii/S0944711322004184

Citation 11

https://www.mdpi.com/1964258

Citation 12

https://www.mdpi.com/1422-0067/23/22/13755

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma.