QuicKey Pro Human αFP (Alpha-Fetoprotein) ELISA Kit

Specifications

Catalog number

E-OSEL-H0015-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ΑFP

UniProt

P02771

Cross Reactivity

This kit recognizes Human αFP in samples.No significant cross-reactivity or interference between Human αFP and analogues was observed

Target

AFP; Alpha-Fetoprotein

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Developmental biology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human αFP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human αFP are added to the micro ELISA plate wells. Human αFP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human αFP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human αFP. The concentration of Human αFP in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human αFP concentrations in serum, plasma.