QuicKey Pro Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit

Specifications

Catalog number

E-OSEL-H0018-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ICAM-1

UniProt

P05362

Cross Reactivity

This kit recognizes Human ICAM-1 in samples. No significant cross-reactivity or interference between Human ICAM-1 and analogues was observed.

Target

CD54

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human ICAM-1 are added to the micro ELISA plate wells. Human ICAM-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ICAM-1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human ICAM-1. The concentration of Human ICAM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.25-16 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.15 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human ICAM-1 concentrations in serum, plasma and other biological fluids.