QuicKey Pro Mouse Pg (Progesterone) ELISA Kit

Specifications

Catalog number

E-OSEL-M0006-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Pg

Cross Reactivity

This kit recognizes Mouse Pg in samples.No significant cross-reactivity or interference between Mouse Pg and analogues was observed

Target

Progesterone; Pg

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Pg are added to the micro ELISA plate wells. Mouse Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

24.97 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2213231721000021

Citation 02

https://www.sciencedirect.com/science/article/pii/S0304389421017829

Citation 03

https://www.sciencedirect.com/science/article/pii/S0269749122016499

Citation 04

https://www.dovepress.com/biocompatible-assessment-of-erythrocyte-membrane-camouflaged-polymeric-peer-reviewed-fulltext-article-IJN

Citation 05

https://www.sciencedirect.com/science/article/pii/S0147651322007679

Citation 06

https://www.sciencedirect.com/science/article/pii/S0160412020322741

Citation 07

https://www.sciencedirect.com/science/article/pii/S0160412020322534

Citation 08

https://www.sciencedirect.com/science/article/pii/S0269749119332531

Citation 09

https://rbej.biomedcentral.com/articles/10.1186/s12958-021-00702-7

Citation 10

https://academic.oup.com/metallomics/advance-article-abstract/doi/10.1093/mtomcs/mfac062/6674774

Citation 11

https://www.sciencedirect.com/science/article/pii/S0378874121008382

Citation 12

https://academic.oup.com/biolreprod/article-abstract/104/6/1347/6161978

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse Pg concentrations in serum, plasma.