QuicKey Pro Mouse IgA (Immunoglobulin A) ELISA Kit

Specifications

Catalog number

E-OSEL-M0007-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IgA

Cross Reactivity

This kit recognizes Mouse IgA in samples.No significant cross-reactivity or interference between Mouse IgA and analogues was observed

Target

Immunoglobulin A; IgA

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgA are added to the micro ELISA plate wells. Mouse IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgA.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgA. The concentration of Mouse IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8812323/

Citation 02

https://www.nature.com/articles/srep34079

Citation 03

https://pubs.rsc.org/en/content/articlehtml/2020/fo/d0fo01841d

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.12173

Citation 05

https://www.sciencedirect.com/science/article/pii/S1756464622000901

Citation 06

https://www.mdpi.com/1603134

Citation 07

https://www.sciencedirect.com/science/article/pii/S1756464620304710

Citation 08

https://www.sciencedirect.com/science/article/pii/S0882401019300622

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IgA concentrations in serum, plasma.