QuicKey Pro Rat T (Testosterone) ELISA Kit

Specifications

Catalog number

E-OSEL-R0003-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

T

Cross Reactivity

This kit recognizes Rat T in samples.No significant cross-reactivity or interference between Rat T and analogues was observed

Target

Testosterone; T

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction; Signal Transduction; Epigenetics and Nuclear Signaling; Developmental Biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat T are added to the micro ELISA plate wells. Rat T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat T in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.07 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0144861722002089

Citation 02

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 03

https://www.mdpi.com/2076-3921/11/10/1879

Citation 04

https://www.sciencedirect.com/science/article/pii/S0147651322010892

Citation 05

https://www.hindawi.com/journals/omcl/2022/2113293/

Citation 06

https://www.sciencedirect.com/science/article/pii/S026974911935955X

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8439717/

Citation 08

https://www.sciencedirect.com/science/article/pii/S0753332222002591

Citation 09

https://www.hindawi.com/journals/omcl/2021/7382900/

Citation 10

https://europepmc.org/article/pmc/pmc9520912?javascript_support=no

Citation 11

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo01744f

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7993663/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat T concentrations in serum, plasma.