QuicKey Pro Rat IgG (Immunoglobulin G) ELISA Kit

Specifications

Catalog number

E-OSEL-R0005-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IgG

Cross Reactivity

This kit recognizes Rat IgG in samples.No significant cross-reactivity or interference between Rat IgG and analogues was observed

Target

Immunoglobulin G; IgG

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IgG are added to the micro ELISA plate wells. Rat IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IgG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat IgG. The concentration of Rat IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

7.81-500 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

2.98 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://europepmc.org/article/pmc/pmc9461140?javascript_support=no

Citation 02

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.201800102RR

Citation 03

https://www.nature.com/articles/srep31771

Citation 04

https://journals.sagepub.com/doi/abs/10.1177/15593258221123672

Citation 05

https://link.springer.com/article/10.1631/jzus.B1800475

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat IgG concentrations in serum, plasma.