QuicKey Pro Rat ADP/Acrp30 (Adiponectin) ELISA Kit

Specifications

Catalog number

E-OSEL-R0006-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

ADP; Acrp30

UniProt

A0A0G2K845

Cross Reactivity

This kit recognizes Rat ADP/Acrp30 in samples.No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed

Target

Adiponectin; GBP28; ACDC; APM1; ADPN; AdipoQ; ADIPQTL1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ADP/Acrp30 are added to the micro ELISA plate wells. Rat ADP/Acrp30 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ADP/Acrp30.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. The concentration of Rat ADP/Acrp30 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

31.25-1000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

6.43 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.mdpi.com/1999-4923/14/7/1368

Citation 02

https://www.mdpi.com/1871912

Citation 03

https://www.mdpi.com/1414304

Citation 04

https://www.mdpi.com/989394

Citation 05

https://www.mdpi.com/2076-3921/9/11/1073

Citation 06

https://www.mdpi.com/1422-0067/22/10/5400

Citation 07

https://pubmed.ncbi.nlm.nih.gov/34305591/

Citation 08

https://www.frontiersin.org/articles/10.3389/fcimb.2021.740236/full

Citation 09

https://www.sciencedirect.com/science/article/pii/S0960076022001303

Citation 10

https://www.sciencedirect.com/science/article/pii/S0899900722003264

Citation 11

https://www.mdpi.com/2131052

Citation 12

https://www.sciencedirect.com/science/article/pii/S0955286320304903

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in serum, plasma.