QuicKey Pro Rat IL-1R2 (Interleukin 1 Receptor Type II) ELISA Kit

Specifications

Catalog number

E-OSEL-R0008-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

IL-1R2

UniProt

P43303

Cross Reactivity

This kit recognizes Rat IL-1R2 in samples.No significant cross-reactivity or interference between Rat IL-1R2 and analogues was observed

Target

IL1R2; CD121b; CDw121b; IL-1R-2; IL-1RT-2; IL-1RT2; IL1R2c; IL1RB

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cardiovascular; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1R2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IL-1R2 are added to the micro ELISA plate wells. Rat IL-1R2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IL-1R2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat IL-1R2. The concentration of Rat IL-1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.nature.com/articles/s41419-022-04533-1

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat IL-1R2 concentrations in serum, plasma.