QuicKey Pro Sheep Cortisol ELISA Kit

Specifications

Catalog number

E-OSEL-S0002-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

Cortisol

Cross Reactivity

This kit recognizes Sheep Cortisol in samples.No significant cross-reactivity or interference between Sheep Cortisol and analogues was observed

Target

11β;17α;21-Trihydroxypregn-4-ene-3;20-dione

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cell biology; Metabolism

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep Cortisol are added to the micro ELISA plate wells. Sheep Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Sheep Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

3.13-200 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.13 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0031938422000889

Citation 02

https://www.mdpi.com/1564546

Species

Sheep

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Sheep Cortisol concentrations in serum, plasma.