QuicKey Pro Sheep T (Testosterone) ELISA Kit

Specifications

Catalog number

E-OSEL-S0003-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

T

Cross Reactivity

This kit recognizes Sheep T in samples.No significant cross-reactivity or interference between Sheep T and analogues was observed

Target

Testosterone; T

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Signal Transduction; Signal Transduction; Epigenetics and Nuclear Signaling; Developmental Biology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep T are added to the micro ELISA plate wells. Sheep T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Sheep T in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

1 h 30 min

Sample Type

Serum, plasma

Detection Range

0.63-40 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.25 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,6 months

Species

Sheep

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Sheep T concentrations in serum, plasma.