Human LEP (Leptin) CLIA Kit

Specifications

Catalog number

E-CL-H0112-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

LEP

UniProt

P41159

Cross Reactivity

This kit recognizes Human LEP in samples.No significant cross-reactivity or interference between Human LEP and analogues was observed

Target

LEPD; OB; OBS; Obesity Homolog

Type

Sandwich-ELISA

Applications

CLIA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

Assay Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the RLU value of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,6 months

Citation 01

https://www.mdpi.com/1660-4601/19/4/2254

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human LEP concentrations in serum, plasma and other biological fluids.