Human HLA-B27 (Human Leukocyte Antigen B27) CLIA Kit

Specifications

Catalog number

E-CL-H0147-01

Size / count

24 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

HLA-B27

UniProt

P01889

Cross Reactivity

This kit recognizes Human HLA-B27 in samples.No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed

Target

Major Histocompatibility Complex (human) ; Class I; B27

Type

Sandwich-ELISA

Applications

CLIA

Field of Research

Immunology

Assay Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HLA-B27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HLA-B27, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HLA-B27. You can calculate the concentration of Human HLA-B27 in the samples by comparing the RLU value of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,6 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human HLA-B27 concentrations in serum, plasma and other biological fluids.